The Biosphère is a museum in Montreal dedicated to the environment. It is located at Parc Jean-Drapeau, on Île Sainte-Hélène in the former pavilion of the United States for the 1967 World Fair Expo 67. The architect of the geodesic dome was Buckminster Fuller. The building originally formed an enclosed structure of steel and acrylic cells, 76 metres in diameter and 62 metres high. The dome is a Class 1, Frequency 16 Icosahedron. A complex system of shades was used to control its internal temperature.

Architects from Golden Metak Productions designed the interior exhibition space. Visitors had access to four themed platforms divided into seven levels. The building included a 37-metre-long escalator, the longest ever built at the time. The Minirail monorail ran through the pavilion. In August, 1990, Environment Canada purchased the site for $17.5 million to turn it into an interactive museum showcasing and exploring the water ecosystems of the Great Lakes-Saint Lawrence River regions. The museum was inaugurated in 1995 as a water museum, and is a set of enclosed buildings designed by Éric Gauthier, inside the original steel skeleton. The Biosphère changed its name in 2007 to become an environment museum. It offers interactive activities and presents exhibitions about the major environmental issues related to water, climate change, air, ecotechnologies and sustainable development.